Dimethyladenosine transferase 1, mitochondrial; Transcription factor B1, mitochondrial is a mitochondrial enzyme that is encoded by the TFB1M gene.[1][2][3]

TFB1M is a mitochondrial methyltransferase, which uses S-adenosyl methionine to dimethylate two highly conserved adenosine residues at the 3'-end of the mitochondrial 12S rRNA thereby regulating the assembly or stability of the small subunit of the mitochondrial ribosome.[2][4][5]

Additionally, TFB1M has been demonstrated to stimulate transcription from promoter templates in an in vitro system containing recombinant mitochondrial RNA polymerase and TFAM.[6] There are no experimental data demonstrating that this function occurs in vivo; the paralogous TFB2M is more specific for this role.[7]

Interactions

TFB1M has been shown to interact with TFAM.[8]

References

  1. ^ Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W (Aug 2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". Genome Res. 10 (5): 703–13. doi:10.1101/gr.10.5.703. PMC 310876. PMID 10810093
  2. ^ McCulloch V, Seidel-Rogol BL, Shadel GS (Jan 2002). "A human mitochondrial transcription factor is related to RNA adenine methyltransferases and binds S-adenosylmethionine". Mol Cell Biol. 22 (4): 1116–25. doi:10.1128/MCB.22.4.1116-1125.2002. PMC 134642. PMID 11809803
  3. ^ "Entrez Gene: TFB1M transcription factor B1, mitochondrial"
  4. ^ Falkenberg M, Gaspari M, Rantanen A, etal (2002). "Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA.". Nat. Genet.. 31 (3): 289–94. doi:10.1038/ng909. PMID 12068295. S2CID 11164308
  5. ^ Metodiev MD; Lesko N; Park CB; Camara Y.; Shi Y.; Wibom R.; Hultenby K.; Gustafsson CM; Larsson NG. (Apr 2009). "Methylation of 12S rRNA is necessary for in vivo stability of the small subunit of the mammalian mitochondrial ribosome". Cell Metab.. 9 (4): 386–97. doi:10.1016/j.cmet.2009.03.001. PMID 19356719
  6. ^ Falkenberg M, Gaspari M, Rantanen A, etal (2002). "Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA.". Nat. Genet.. 31 (3): 289–94. doi:10.1038/ng909. PMID 12068295. S2CID 11164308
  7. ^ Hillen, HS; Morozov, YI; Sarfallah, A; Temiakov, D; Cramer, P (16 November 2017). "Structural Basis of Mitochondrial Transcription Initiation.". Cell. 171 (5): 1072–1081.e10. doi:10.1016/j.cell.2017.10.036. PMC 6590061. PMID 29149603
  8. ^ McCulloch, Vicki & Shadel Gerald S (Aug 2003). "Human mitochondrial transcription factor B1 interacts with the C-terminal activation region of h-mtTFA and stimulates transcription independently of its RNA methyltransferase activity". Mol. Cell. Biol.. 23 (16): 5816–24. doi:10.1128/MCB.23.16.5816-5824.2003. ISSN 0270-7306. PMC 166325. PMID 12897151

Further reading